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Armed with this particular facts, It could be most enlightening to trace how archaeal enzymes from closely connected taxa which have really related Main constructions arrived to vary inside their nucleotide specificities, culminating by introducing amino acids adjustments that elicit a get of perform, converting an ATP-only DNA ligase into a dual-specificity by-product.
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). The NTase area binds to your broken and intact DNA strands in the key groove flanking the nick as well as during the slight groove on the 3′-OH side with the nick (supplemental Fig. S3). The OB area binds across the slight groove to the experience from the duplex guiding the nick.
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